Figure 4

Comparing expression of genes through microarray and QRT-PCR assays. We used real-time PCR analysis to confirm the differential expression of 14 genes of the African strain MAI1 of Xanthomonas oryzae pv. oryzae. The genes represented various biological functional classes of interest. Although fold change in gene expression was generally higher for QRT-PCR than for the microarray, good correlation existed between the two data sets. A subset of 5 genes corresponding to xopX (ACD57163), HrpF protein (FI978263), hypothetical proteins (FI978252 and FI978328), and the avrXa7 gene (AF275267) showing a fold-change higher than 10 in QRT-PCR is illustrated in the figure. Three replicates were analysed in both microarray and QRT-PCR experiments. Vertical bars represent standard deviations.