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Table 1 Strains and plasmids used in this study

From: Bicarbonate enhances expression of the endocarditis and biofilm associated pilus locus, ebpR-ebpABC, in Enterococcus faecalis

Strain or Plasmid Relevant characteristics Source or reference
E. coli strains   
   TG1 E. coli general cloning host [35]
E. faecalis strains   
   OG1RF E. faecalis. FusR, RifR [36]
   TX5266 OG1RF fsrB deletion mutant, deletion from bp 79 to 684 of fsrB. FusR, RifR [6]
   TX5514 OG1RF ebpR deletion mutant, deletion from -5 bp to +1337 bp of ebpR. FusR, RifR [11]
   TX5584 TX5514(pMSP3535). ErmR, FusR, RifR [11]
   TX5582 TX5514(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR [11]
   TX5583 OG1RF(pMSP3535). ErmR, FusR, RifR This study
   TX5581 OG1RF(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR This study
Plasmids   
   pTCV-lacZ Shuttle vector containing promoterless lacZ. ErmR [32]
   pMSP3535 Nisin inducible expression shuttle vector with pAMβ 1 and ColE1 replicons. ErmR [37]
   pTEX5269 fsrB promoter cloned upstream of lacZ in pTCV-lacZ (P fsrB ::lacZ), from bp -110 to -8 (103 bp) relative to fsrB start codon; ErmR [6]
   pTEX5585 ebpA promoter cloned upstream of lacZ in pTCV-lacZ (P ebpA ::lacZ), from -221 bp to +80 bp (301 bp) relative to ebpA start codon. ErmR This study
   pTEX5586 ebpR promoter cloned upstream of lacZ in pTCV-lacZ (P ebpR ::lacZ), from -248 to + 53 bp (301 bp) relative to ebpR start codon. ErmR [11]
   pTEX5515 pMSP3535 with ebpR from -20 bp to +1561 bp from the ATG. This ebpR fragment contains the full ORF and the RBS of ebpR. ErmR [11]