Bicarbonate enhances expression of the endocarditis and biofilm associated pilus locus, ebpR-ebpABC , in Enterococcus faecalis

Table 1 Strains and plasmids used in this study

Strain or Plasmid	Relevant characteristics	Source or reference
E. coli strains
TG1	E. coli general cloning host	[35]
E. faecalis strains
OG1RF	E. faecalis. Fus^R, Rif^R	[36]
TX5266	OG1RF fsrB deletion mutant, deletion from bp 79 to 684 of fsrB. Fus^R, Rif^R	[6]
TX5514	OG1RF ebpR deletion mutant, deletion from -5 bp to +1337 bp of ebpR. Fus^R, Rif^R	[11]
TX5584	TX5514(pMSP3535). Erm^R, Fus^R, Rif^R	[11]
TX5582	TX5514(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. Erm^R, Fus^R , Rif^R	[11]
TX5583	OG1RF(pMSP3535). Erm^R, Fus^R, Rif^R	This study
TX5581	OG1RF(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. Erm^R, Fus^R , Rif^R	This study
Plasmids
pTCV-lacZ	Shuttle vector containing promoterless lacZ. Erm^R	[32]
pMSP3535	Nisin inducible expression shuttle vector with pAMβ 1 and ColE1 replicons. Erm^R	[37]
pTEX5269	fsrB promoter cloned upstream of lacZ in pTCV-lacZ (P_fsrB::lacZ), from bp -110 to -8 (103 bp) relative to fsrB start codon; Erm^R	[6]
pTEX5585	ebpA promoter cloned upstream of lacZ in pTCV-lacZ (P_ebpA::lacZ), from -221 bp to +80 bp (301 bp) relative to ebpA start codon. Erm^R	This study
pTEX5586	ebpR promoter cloned upstream of lacZ in pTCV-lacZ (P_ebpR::lacZ), from -248 to + 53 bp (301 bp) relative to ebpR start codon. Erm^R	[11]
pTEX5515	pMSP3535 with ebpR from -20 bp to +1561 bp from the ATG. This ebpR fragment contains the full ORF and the RBS of ebpR. Erm^R	[11]

ISSN: 1471-2180