Figure 5

ebpR and ebpA expression profiles in TX5266 (Δ fsrB mutant). For β-gal assays, samples were collected every hour from 3 to 8 hr, then at 10 and 24 hr after starting the culture (x axis). The left axis represents the β-gal units (OD_420nm/protein concentration in mg/ml). The right axis indicates the OD_{600 nm} readings. All sets of cultures presented were analyzed concurrently. Each figure is a representative of at least three experiments. A. OG1RF containing either P _ebpR ::lacZ (black triangle) or P _ebpA ::lacZ (black square) and ΔfsrB containing either P _ebpR ::lacZ (pink triangle) or P _ebpA ::lacZ (pink square) were grown in TSBG aerobically. B. The ΔfsrB mutant (TX5266) containing either P _ebpR ::lacZ (triangle) or P _ebpA ::lacZ (square) was grown in TSBG aerobically (pink closed symbol) or in the presence of 5% CO₂/0.1 M NaHCO₃ (open blue symbol).