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Table 2 Robust performance of standard control genes using CAB as sole reference to set a manual threshold at 26 Ct and a master equation derived from 80 replicated plate reactions on Applied Biosystems 7500 real time PCR System

From: Quantitative transcription dynamic analysis reveals candidate genes and key regulators for ethanol tolerance in Saccharomyces cerevisiae

Control gene Reference Ct Mean Ct Stdev Estimated mRNA (pg) Input mRNA (pg) Consistency (%)
MSG   29.429 0.077 0.098 0.1 98.1
CAB 26.0 25.965 0.037 0.984 1 98.4
RBS1   22.388 0.019 10.64 10 93.6
ACTB   15.604 0.019 973.25 1000 97.3