Glucose metabolic pathway response. Illustrative pathways of ethanol- and inhibitor-tolerant mutant Saccharomyces cerevisiae NRRL Y-50316 involved in trehalose-glycolysis-pentose phosphate pathway in response to ethanol challenges inferred by dynamic quantitative mRNA expression analysis and metabolic profiling analysis compared with its parental strain NRRL Y-50049. Dark green arrowed lines and letters indicate high levels (5.1-60 fold increase for at least one critical time point) of mRNA expression and enhanced pathways, green for significant levels (1.5-5 fold increase for at least one critical time point) of enhanced transcription and pathways; black indicates normal or nearly normal levels of transcription and pathway events, red for repressed expression, reactions, or pathways. Bold lines and letters indicate the levels of expression and pathways are statistically significant at P < 0.05. Reactions involved in NAD(P)H regeneration steps are circled in blue.