Figure 1

Cell growth response to ethanol and inhibitors. Comparison of cell growth and colony appearance for ethanol-tolerant and inhibitor-tolerant mutant Saccharomyces cerevisiae NRRL Y-50316 and its parental inhibitor-tolerant strain NRRL Y-50049 on YM plate of 2% glucose containing 8% (v/v) ethanol (A) or amended with inhibitors of furfural and 5-hydroxymethylfurfural each at 10 mM (B). The cultures initially applied were estimated with viable cell account of approximately 1.0 × 10⁷ per ml as measured by colony forming units. A serial of 10-fold culture dilutions in water were spotted onto a medium plate containing ethanol or inhibitors and cell growth examined 7 days after incubation at 30°C.