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Figure 1 | BMC Microbiology

Figure 1

From: Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression

Figure 1

Intracellular survival of B. melitensis 16M (wt), vjbR mutant (Δ vjbR and vjbR ::m Tn 5), complemented Δ vjbR (Δ vjbR comp and Δ vjbRvector ), Δ blxR mutant, and 3 additional luxR -like mutants in J774A.1 murine macrophage-like cells. The attenuation was measured as the log difference between the CFU recoveries of the mutant compared to wildtype from infected macrophages at 48 hours post infection. Data shown is the averaged CFU recovery from at least 3 independent experiments, each performed in triplicate. Error bars represent the SEM and each mutant was compared to the wildtype by a Student's two tailed t-test, with the resulting p values as follows:*, P < .0.05; ***, P < 0.001. The luxR deletion mutant strains are identified by the BME gene locus ID tags, BME::Km representing the gene replacement mutant and ΔBME representing the gene deletion mutant.

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BMC Microbiology

ISSN: 1471-2180

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