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Figure 6 | BMC Microbiology

Figure 6

From: Identification of a novel anti-σE factor in Neisseria meningitidis

Figure 6

Residues Cys4, Cys34 and Cys37 of NMB2145 are essential for optimal anti-σE activity of NMB2145. SDS-PAGE assessment of MsrA/MsrB protein levels in crude membranes extracted from ΔNMB2145 cells in which mutant NMB2145 proteins pNMB2145(His30Ala), pNMB2145(Cys4Ala), pNMB2145(Cys34Ala) and pNMB2145(Cys37Ala) are expressed. Crude membranes were extracted before (-) and after (+) induction. Molecular weight markers (kDa) are indicated on the right. Arrow indicates MsrA/MsrB.

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