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Figure 4 | BMC Microbiology

Figure 4

From: Identification of a novel anti-σE factor in Neisseria meningitidis

Figure 4

NMB2145 represses transcription of the rpoE operon. Products obtained by RT-PCR were separated on agarose gel. RT-PCR analysis of transcription of the rpoE operon in the wt strain (H44/76), H44/76 transformed with pNMB2144 before (-) and after (+) induction of expression of rpoE, after deletion of NMB2145 (ΔNMB2145) and before (-) and after (+) induction of NMB2145 in the ΔNMB2145 background (upper panel). RT-PCR was carried out using primer pair 2140-01/2143-02 (cf Fig.1a) and RT-PCR on rmpM (lower panel) was used as input control of total RNA.

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