Table 2 Composition of chemically defined media (CDM)
Reagent | Concentration |
|---|---|
NaCl | 0.1 M |
K2SO4 | 5.75 mM |
Na2EDTA | 4 mM |
NH4Cl | 4 mM |
K2HPO4 | 2 mM |
KH2PO4 | 2 mM |
Thiamine HCl | 6 μM |
Thiamine pyrophosphate | 1 μM |
Pantothenic acid | 8 μM |
d-Biotin | 12 μM |
Glucose | 0.5% |
Hypoxanthine | 0.375 mM |
Uracil | 0.45 mM |
L-aspartic acid | 3.75 mM |
L-glutamic acid HCl | 7.5 mM |
L-arginine | 0.875 mM |
Glycine HCl | 0.225 mM |
L-serine | 0.475 mM |
L-leucine | 0.7 mM |
L-isoleucine | 0.225 mM |
L-valine | 0.525 mM |
L-tyrosine | 0.4 mM |
L-cysteine HCl | 0.35 mM |
L-cystine | 0.15 mM |
L-proline | 0.45 mM |
L-tryptophan | 0.4 mM |
L-threonine | 0.425 mM |
L-phenylalanine | 0.15 mM |
L-asparagine | 0.2 mM |
L-glutamine | 0.35 mM |
L-histidine HCl | 0.125 mM |
L-methionine | 0.1 mM |
L-alanine | 1.125 mM |
L-lysine | 0.35 mM |
Glutathione reduced | 0.15 mM |
HEPES | 42 mM |
NaHCO3 | 0.125 mM |
Na acetate trihydrate | 6.25 mM |
Choline chloride salt | 0.05 mM |
Myo-inositol | 1 μM |
MgCl2 | 2.5 mM |
CaCl2 | 0.6 mM |
Fe(NO3)3 | 0.1 mM |
Nicotinamide adenine dinucleotide | 0.02 mM |
Protoporphyrin IX | 0.02 mM |
Histidine | 6 μM |
Triethanolamine | 0.01% |