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Table 1 Proteins of the glycolysis pathway identified in H. influenzae strain 11P6H identified during growth in pooled human sputum

From: Proteomic expression profiling of Haemophilus influenzae grown in pooled human sputum from adults with chronic obstructive pulmonary disease reveal antioxidant and stress responses

Protein ID#

Identified Protein

Gene

Genome ID numbera

Molecular Weight

CDMb

Sputumc

1237

phosphoenolpyruvate-protein phosphotransferase

fruA

HI0446

64 kDa

100% (9.7%)

100% (11%)

412

Fructose specific phosphotransferase system IIA/HPr components

fruB

HI0448

53 kDa

100% (24%)

100% (8.2%)

1149

Aldose 1-epimerase

galM

HI0818

38 kDa

100% (11%)

100% (15%)

423

1-phosphofructokinase

fruK

HI0447

34 kDa

100% (24%)

100% (15%)

557

6-phosphofructokinase

pfkA

HI0982

23 kDa

100% (21%)

95% (20%)

57

Fructose-bisphosphate aldolase

fba

HI0524

39 kDa

100% (47%)

100% (36%)

657

glucose-6-phosphate isomerase

pgi

HI1576

37 kDa

100% (19%)

100% (15%)

392

Triosephosphate isomerase

tplA

HI0678

27 kDa

100% (25%)

100% (19%)

97

Glyceraldehyde-3-phosphate dehydrogenase

gapA

HI0001

36 kDa

100% (40%)

100% (39%)

111

3-phosphoglycerate kinase

pgk

HI0525

41 kDa

100% (39%)

100% (37%)

34

phosphoglyceromutase

gpmA

HI0757

26 kDa

100% (52%)

100% (56%)

79

enolase

eno

HI0932

46 kDa

100% (43%)

100% (32%)

133

Pyruvate kinase

pykA

HI1573

49 kDa

100% (37%)

100% (47%)

538

Dihydrolipoamide acetyltransferase

aceF

HI1232

57 kDa

100% (22%)

100% (23%)

305

Pyruvate dehydrogenase subunit E1

aceE

HI1233

99 kDa

100% (28%)

100% (30%)

  1. aID numbers based on annotation of H. influenzae strain KW20 Rd http://cmr.jcvi.org/cgi-bin/CMR/GenomePage.cgi?org=ghi
  2. bProtein probabilities values as calculated by Proteinprophet algorithm for proteins detected during growth in chemically define media (CDM). Number in parentheses represents the sequence coverage expressed by the percentage of amino acid residues identified. All peptides were filtered with a set of criteria as specified in the Methods. CDM
  3. cProtein probabilities for proteins detected during growth in 20% pooled human sputum.