Development of a versatile tool for the simultaneous differential detection of Pseudomonas savastanoi pathovars by End Point and Real-Time PCR

Table 1 Bacteria used in this study.

Strain^a	Host plant of isolation	Geographical origin	End Point PCR			Real-Time PCR
*P.savastanoi* pv. *savastanoi*			pathovar- specific primer pairs			pathovar- specific primers/probes
			*Psv*	*Psn*	*Psf*	*Psv* -RT	*Psn* -RT	*Psf* -RT
ITM317, IPVCT-3, LPVM22, LPVM510, LPVM602, ES47^b, ES49^b, ESB50^b, PvBa223	olive	Southern Italy	+	-	-	+	-	-
Legri1^b, Legri2^b, MC1^b, MC33^b, MC72^b, MC80^b, LPVM15, LPVM20	olive	Central Italy	+	-	-	+	-	-
ITMKS1, ITMKL1, ST2^b	olive	Greece	+	-	-	+	-	-
1657-8^c	olive	Spain	+	-	-	+	-	-
DAR7635^d	olive	Australia	+	-	-	+	-	-
P. savastanoi pv. nerii
ITM519, IPVCT-99, ESC8^b, ESC6^b, ESC43^b, ESB60^b, LPVM12, LPVM33, LPVM71, LPVM201, PvBa219	oleander	Southern Italy	-	+	-	-	+	-
ITM601, ES23^b, LPVM103	oleander	Northern Italy	-	+	-	-	+	-
NCPPB640	oleander	Ex-Yugoslavia	-	+	-	-	+	-
P. savastanoi pv. fraxini
NCPPB1006, NCPPB1464	ash	United Kingdom	-	-	+	-	-	+
PD120	ash	The Netherlands	-	-	+	-	-	+
CFBP1838, CFBP2093	ash	France	-	-	+	-	-	+
MCa3^b, MCa4^b	ash	Italy	-	-	+	-	-	+
P. savastanoi pv. phaseolicola 1449B^e	Lablab purpureus	Ethiopia	-	-	-	-	-	-
P. savastanoi pv. glycinea PG4180^f	soybean	New Zealand	-	-	-	-	-	-
Unidentified bacteria^g	olive, oleander, ash	Central Italy	-	-	-	-	-	-

^a CFBP, Collection Française de Bactéries Phytopathogènes, INRA, Angers, France; IPVCT, Culture collection of Dipartimento di Scienze e Tecnologie Fitosanitarie, Università degli Studi di Catania, Italy (from V. Catara); ITM, Culture collection of Istituto Tossine e Micotossine da Parassiti vegetali, C. N. R., Bari, Italy (from A. Sisto); LPVM, Culture Collection of Laboratorio di Patologia Vegetale Molecolare, Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze; NCPPB, National Collection of Plant Pathogenic Bacteria, York, UK http://www.ncppb.com/; PD, Culture collection of Plant Protection Service, Wageningen, The Netherlands; PVBa, Culture Collection of Dipartimento di Patologia Vegetale, Università degli Studi di Bari, Italy (from A. Sisto).
^b from E. Santilli and M. Cerboneschi
^c from M. M. Lopez
^d from E. J. Cother
^e from R. W. Jackson
^f from M. S. Ullrich
^g bacterial epiphytes naturally occurring P. savastanoi host plants and isolated as described in Methods.

ISSN: 1471-2180