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Figure 5 | BMC Microbiology

Figure 5

From: Development of a versatile tool for the simultaneous differential detection of Pseudomonas savastanoi pathovars by End Point and Real-Time PCR

Figure 5

Sensitivity of TaqMan® probes in Multiplex Real-Time PCR assays. Sensitivity of the TaqMan® probes Psv RT-P, Psn RT-P and Psf RT-P was evaluated using P. savastanoi DNA extracted from olive leaves artificially inoculated with bacterial suspensions (107 CFU/leaf/strain) of Psv ITM317 (red triangle), Psn ITM519 (green triangle) and Psf NCPPB1464 (blue triangle), according to the following scheme. (A) Psv ITM317; (B) Psv ITM317 + Psn ITM519; (C) Psv ITM317 + Psn ITM519 + Psf NCPPB1464. Amplification curves obtained with DNA from Psv ITM317 (red diamond), Psn ITM519 (green diamond) and Psf NCPPB1464 (blue diamond) (50 ng/reaction each) and from water and uninoculated leaves (-) were also shown for comparison. (See online for a colour version).

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