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Figure 4 | BMC Microbiology

Figure 4

From: Development of a versatile tool for the simultaneous differential detection of Pseudomonas savastanoi pathovars by End Point and Real-Time PCR

Figure 4

Sensitivity of TaqMan® probes Psv RT-P (A), Psn RT-P (B) and Psf RT-P (C). Sensitivity was assessed by using DNA extracted from strains Psv ITM317 (A), Psn ITM519 (B) and Psf NCPPB1464 (C). Amplification curves of DNA from target P. savastanoi pathovar extracted from 103, 105 and 107 CFU per reaction and used pure (red diamond, green diamond and blue diamond, respectively) or spiked with no-target P. savastanoi pathovars DNA (50 ng/reaction each) (black diamond) or with DNA from the host plant of target P. savastanoi pathovar and from a pool of bacterial epiphytes present on this plant (50 ng/reaction each) (grey square). (See online for a colour version of this figure). Standard curves were generated by plotting the Ct values versus the log of genomic DNA concentration of each tenfold dilution series in the range of linearity (from 50 ng to 0.5 pg per reaction). The Ct data obtained with target DNA from 103 to 107 CFU per reaction were reported (X). (See online for a colour version of this figure).

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BMC Microbiology

ISSN: 1471-2180

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