Figure 2

Specificity and detection limit of End Point PCR assays.(A) primer set Psv F/Psv R on strain Psv ITM317; (B) primer set Psn F/Psn R on strain Psn ITM519; (C) primer set Psf F/Psf R on strain Psf NCPPB1464. M, marker 1 Kb Plus Ladder (Invitrogen Inc.). lanes 1-7: genomic DNA from the target P. savastanoi pathovar (serial tenfold dilutions, from 50 ng to 0.05 pg per reaction); lanes 8-9: genomic DNA from the non-target P. savastanoi pathovars (50 ng/reaction); lanes 10-13: plant genomic DNA (50 ng/reaction), from olive, oleander, ash and oak, respectively; lane 14: genomic DNA (50 ng/reaction) from a pool of bacterial epiphytes isolated in this study from olive (A), oleander (B) and ash leaves (C); lane 15, DNA-free negative control;