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Table 1 Oligonucleotides used in this study

From: Natural transformation of Vibrio cholerae as a tool - Optimizing the procedure

Primer name Sequence
Nhe I-lacZ-start 5'-PCGCGCTAGCAAAGGCGTTATTGGCTTGTTGC-3'
LacZ-end-Sal I 5'-PCGCGTCGACGCTTTCACACGTAAGGTGAGC-3'
Tfm-II-1000 5'-CGGGAAGCTAGAGTAAGTAGTTCG-3'
Tfm-II+1000 5'-CGTTCCATGTGCTCGCCGAGGCG-3'
Tfm-II-gDNA-1000 5'-AAGCTTCCTGCTTGGAAGAAATGGC-3
Tfm-II-gDNA+1000 5'-CGGTGTATCTGTGGCAACGGTTTC-3'
Tfm-II-2000 5'-CCCCCCTGACGAGCATCACAAAAATCG-3'
Tfm-II+2000 5'-CTGACGCGCCCTGACGGGCTTGTCTGC-3'
Tfm-II-gDNA-2000 5'-GAAACCGACGAAGGTGTGTTGATC-3'
Tfm-II-gDNA+2000 5'-CGCAACCGGATTGGTGCGCTATTTTGGC-3'
KanR-500flank-up 5'-GCGCTTTATCAACACGCTGAATTGC-3'
KanR-500flank-down 5'-ACGCGAAGATCGTCACATTCCACAC-3'
KanR-250flank-up 5'-TGCTTGATGAAGATGGCGCGCCG-3'
KanR-250flank-down 5'-CATCTTGCTGCCATTGAGGCAGCG-3'
KanR-100flank-up 5'-ATGTGATGGATGAAGCAAGCATGCG-3'
KanR-100flank-down 5'-ATTCATGCTCTGGCAACATTGGCAGC-3'