Table 1 Oligonucleotides used in this study
From: Natural transformation of Vibrio cholerae as a tool - Optimizing the procedure
Primer name | Sequence |
|---|---|
Nhe I-lacZ-start | 5'-PCGCGCTAGCAAAGGCGTTATTGGCTTGTTGC-3' |
LacZ-end-Sal I | 5'-PCGCGTCGACGCTTTCACACGTAAGGTGAGC-3' |
Tfm-II-1000 | 5'-CGGGAAGCTAGAGTAAGTAGTTCG-3' |
Tfm-II+1000 | 5'-CGTTCCATGTGCTCGCCGAGGCG-3' |
Tfm-II-gDNA-1000 | 5'-AAGCTTCCTGCTTGGAAGAAATGGC-3 |
Tfm-II-gDNA+1000 | 5'-CGGTGTATCTGTGGCAACGGTTTC-3' |
Tfm-II-2000 | 5'-CCCCCCTGACGAGCATCACAAAAATCG-3' |
Tfm-II+2000 | 5'-CTGACGCGCCCTGACGGGCTTGTCTGC-3' |
Tfm-II-gDNA-2000 | 5'-GAAACCGACGAAGGTGTGTTGATC-3' |
Tfm-II-gDNA+2000 | 5'-CGCAACCGGATTGGTGCGCTATTTTGGC-3' |
KanR-500flank-up | 5'-GCGCTTTATCAACACGCTGAATTGC-3' |
KanR-500flank-down | 5'-ACGCGAAGATCGTCACATTCCACAC-3' |
KanR-250flank-up | 5'-TGCTTGATGAAGATGGCGCGCCG-3' |
KanR-250flank-down | 5'-CATCTTGCTGCCATTGAGGCAGCG-3' |
KanR-100flank-up | 5'-ATGTGATGGATGAAGCAAGCATGCG-3' |
KanR-100flank-down | 5'-ATTCATGCTCTGGCAACATTGGCAGC-3' |