Figure 4
From: Natural transformation of Vibrio cholerae as a tool - Optimizing the procedure
Induction of natural competence by different chitin sources. Different chitin sources and chitin derivatives were tested for their ability to induce natural competence in V. cholerae A1552. Lanes 1 and 2: chitosan; lanes 3 and 4: chitin flakes; lanes 5 and 6: chitin powder; lanes 7 and 8: crab-shell fragments (approx. 1 cm2). The medium was not changed at the time of donor DNA (2 ug LacZ-Kan gDNA) addition for all odd lanes but for all even lanes. Average of four independent experiments.