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Figure 4 | BMC Microbiology

Figure 4

From: Natural transformation of Vibrio cholerae as a tool - Optimizing the procedure

Figure 4

Induction of natural competence by different chitin sources. Different chitin sources and chitin derivatives were tested for their ability to induce natural competence in V. cholerae A1552. Lanes 1 and 2: chitosan; lanes 3 and 4: chitin flakes; lanes 5 and 6: chitin powder; lanes 7 and 8: crab-shell fragments (approx. 1 cm2). The medium was not changed at the time of donor DNA (2 ug LacZ-Kan gDNA) addition for all odd lanes but for all even lanes. Average of four independent experiments.

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