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Table 3 Quantification of ICEclc core gene expression by dot-blot hybridization in strain B13 grown on different carbon substrates.

From: Transcriptome analysis of the mobile genome ICEclc in Pseudomonas knackmussii B13

 

Exponential phase

After 24 h at stationary phase

 

3-chlorobenzoate

succinate

3-chlorobenzoate

succinate

fructose

glucose

Probe number and probe

mRNAa

Std Devb

mRNA

Std Dev

mRNA

Std Dev

mRNA

Std Dev

mRNA

Std Dev

mRNA

Std Dev

  

(%)

 

(%)

 

(%)

 

(%)

 

(%)

 

(%)

1) intB13

4.5

11.2

7.3

13.1

5.1

28.5

4.1

11.2

4.4

51.7

3.2

8.1

2) ORF52710

21.3

46.5

19.7

16.9

9.3

39.9

9.6

8

5.1

42.7

9.4

30.6

3) ORF53587

4.2

30.2

3.6

0.1

1.7

37.3

1.7

21.1

2

0.4

1.9

2.6

4) ORF59888

18.6

33

16.9

2.3

8.4

32.3

12.9

18.6

16.8

7.3

23.8

15.9

5) ORF65513

17.3

19.4

19.5

2.8

13.4

9.9

12.7 ‡

5.3

13.8

7.6

13.8

11

6) ORF67800

16.6

2.7

16.6

5.5

8

12.9

12.7

18.3

11.6

33.7

17.9

38.6

7) ORF68987

2.1

4.3

2.1

11

0.8

12.9

0.8

0.2

1.3

13.8

1.1

11.7

8) ORF73029

2.5

20.8

2.9

12.6

2.6*

15

0.9 ‡

18.2

1.4

6.7

1.1

4.2

9) ORF75419

7.5

18.1

7.3

6.8

11.1

32

3 ‡

3.9

3.9

3.3

2.8

5.4

10) ORF81655

10.2

30.1

18.7

36.6

168*

24.5

6.3

2.7

45.7*

3.6

9.2

27

11) ORF83350

3.3

18.9

2.8

16.5

0.9

26.1

0.5

37.3

0.5

14.5

0.4

10.2

12) ORF84835

0.4

14.4

0.3

16.3

9.5*

7.7

0.3

25.8

1.7

16.1

0.3

1.5

13) ORF87986

5

1

5.2

0.1

64.5*

7.2

5.5 ‡

0.4

14.2

26.9

5.5

2

14) ORF89746

12.9

34.1

24.4

19.8

2.2

41.2

2.1

17.3

2.1

36.7

0.5

15.4

15) ORF91884

3.3

11.7

4.5

3

3

32.4

1.6 ‡

3.2

2.3

33.1

1.1

5.9

16) inrR

8.3

11.9

8.2

21.3

4.5

11.6

4

7.5

6.4

8.1

4.9

39.7

17) ORF96323

3

13

5.3

27.1

1

2.8

1.8

35.6

0.9

53.2

1.1

31.8

18) ORF98147

1.1

10.7

1.5

5.1

0.5

5.9

0.4 ‡

7

0.4

3.7

0.4

1.7

19) ORF100033

30.6

4

40.4

20.2

12.3

16.7

17.6

18

22.9

6.4

22.2

30.2

20) ORF100952

1.4

13.2

2.2

22.2

1.8 §

3.1

0.9

1.7

1.9

7.9

0.9

29.4

21) ORF101284

3.7

18.9

3.2

6.9

1

23.1

1 ‡

7.9

1.1

1.9

1.2

14.5

  1. a) mRNA is the average calculated amount of mRNA copies × 108 per μg of total RNA from triplicate determinations. This value corresponds approximately to the calculated number of mRNA of this transcript per cell.
  2. b) standard deviation from the average mRNA, expressed in percentage.
  3. c) *, values statistically significantly different to the cells cultivated with other carbon sources. §, values statistically significantly different to the cells cultivated with succinate or glucose. ‡, values statistically significantly different between exponential phase and stationary phase. P ≤ 0.05 in pairwise Student's T test.
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BMC Microbiology

ISSN: 1471-2180

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