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Figure 3 | BMC Microbiology

Figure 3

From: Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization

Figure 3

Identification of the anti-HBP35-immunoreactive 27-kDa protein and the start codons of anti-HBP35-immunoreactive proteins. A. PMF analysis of the anti-HBP35-immunoreactive 27-kDa protein from KDP164 (hbp35 insertion mutant). Underlined peptide fragments were indicated by the PMF data of the protein. Bold letters indicating M115 and M135 were suspected to be internal start codons. B. Immunoblot analysis of P. gingivalis mutants with various amino acid substitutions of HBP35 protein. Lane 1, KDP164 (hbp35 insertion mutant); lane 2, KDP168 (hbp35 [M115A] insertion mutant); lane 3, KDP169 (hbp35 [M135A] insertion mutant); lane 4, KDP170 (hbp35 [M115A M135A] insertion mutant).

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BMC Microbiology

ISSN: 1471-2180

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