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Figure 2 | BMC Microbiology

Figure 2

From: Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization

Figure 2

Subcellular localization of HBP35. Subcellular fractions of P. gingivalis 33277 (lanes 1 to 5) and KDP164 (hbp35 insertion mutant) (lanes 6 to 10) were subjected to immunoblot analysis using anti-HBP35 antibody. Lanes 1 and 6, whole cells; lanes 2 and 7, cytoplasm/periplasm fraction; lanes 3 and 8, total membrane fraction; lanes 4 and 9, inner membrane fraction; lanes 5 and 10, outer membrane fraction. Horizontal lines between lane 5 and 6 indicate the molecular size marker proteins corresponding to the far left markers. Asterisks indicate the non-specific protein bands recognized by anti-HBP35 antibody.

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