Skip to main content

Advertisement

Table 4 Enzyme activity of enzymes and relative expression level of genes for acetate metabolism in pyruvate-grown cultures during phototrophic and chemotrophic growth.

From: Energy metabolism of Heliobacterium modesticaldum during phototrophic and chemotrophic growth

  Specific activity (nmole/min/mg protein)  
Enzyme activity tested Phototrophic growth Chemotrophic growth Relative gene expression level (light/dark)
acetyl-CoA synthetase (ACS)a 100 ± 20 N/A 45
acetate kinase (ACK)a 800 ± 40 600 ± 100 3
phosphotransacetylase (PTA)a 400 ± 50 500 ± 100 --
  1. aActivity of ACS was determined by a colorimetric assay of PPi [39], and activity of ACK and PTA was determined by coupling assays reported previously [18].