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Table 2 Gene expression profiles for phototrophic (light) vs. chemotrophic (dark) growth.

From: Energy metabolism of Heliobacterium modesticaldum during phototrophic and chemotrophic growth

Gene name ΔCTa(light) ΔCTa(dark) ΔΔCTb Relative gene expression level (light/dark)c
Genes for carbon metabolism
pfkA (6-phosphofructokinase) 15.0 ± 0.1 22.0 ± 0.1 7.0 ± 0.2 128
pykA (pyruvate kinase) 13.5 ± 0.1 19.5 ± 0.1 6.0 ± 0.2 64
porA (pyruvate:Fd oxidoreductase) 13.7 ± 0.1 11.6 ± 0.0 -2.1 ± 0.1 0.2
fdxR (Fd-NADP+ oxidoreductase) 14.7 ± 0.1 15.2 ± 0.1 0.5 ± 0.2 1.4
Ferredoxin 13.4 ± 0.1 13.2 ± 0.1 -0.2 ± 0.2 1
pshB (ferredoxin) 14.0 ± 0.1 14.3 ± 0.1 0.3 ± 0.2 1
ackA (acetate kinase) 10.6 ± 0.1 12.2 ± 0.1 1.6 ± 0.2 3
acsA (acetyl-CoA synthase) 15.5 ± 0.1 21.0 ± 0.1 5.5 ± 0.2 45
ppdK (pyruvate phosphate dikinase) 13.4 ± 0.1 17.4 ± 0.1 4.0 ± 0.2 16
pckA (PEP carboxykinase) 14.1 ± 0.1 17.2 ± 0.1 3.1 ± 0.2 8
mdh (malate dehydrogenase) 14.5 ± 0.1 14.6 ± 0.1 0.1 ± 0.2 1
Genes for pigment biosynthesis
bchY 13.1 ± 0.1 15.7 ± 0.0 2.6 ± 0.1 6
bchB 14.0 ± 0.0 18.0 ± 0.1 4.0 ± 0.1 16
bchE 13.2 ± 0.1 15.0 ± 0.1 1.8 ± 0.2 4
bchG 12.9 ± 0.1 13.9 ± 0.1 1.0 ± 0.2 2
Genes for nitrogen assimilation and hydrogen production
nifK (Fe/Mo nitrogenase, β subunit) 13.0 ± 0.0 21.5 ± 0.1 8.5 ± 0.1 365
nifD (Fe/Mo nitrogenase, α subunit) 13.7 ± 0.0 21.4 ± 0.1 7.7 ± 0.1 197
hupS ([NiFe]-hydrogenase small subunit) 13.3 ± 0.1 18.4 ± 0.1 5.1 ± 0.2 34
hupL ([NiFe]-hydrogenase large subunit) 12.7 ± 0.1 18.3 ± 0.1 5.6 ± 0.2 49
hymD (Fe only hydrogenase, Hymd subunit) 13.4 ± 0.1 18.7 ± 0.1 5.3 ± 0.2 40
nuoE 14.3 ± 0.2 19.7 ± 0.1 5.4 ± 0.3 43
nuoF 12.9 ± 0.2 18.6 ± 0.1 5.7 ± 0.3 51
nuoG 12.9 ± 0.1 18.6 ± 0.1 5.7 ± 0.2 51
  1. aΔCT = CT (the threshold cycle) of the target gene - CT of the 16S rRNA gene [31]
  2. bΔΔCT = ΔCT (dark) - ΔCT (light)
  3. c relative expression level is = 2ΔΔCT