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Table 2 Effect of various carbon sources on AThTP production by different E. coli strains.

From: Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress

 

AThTP (pmol/mg of protein)

MG1655

 

Control

62 ± 6

D-Glucose (10 mM)

11 ± 2

L-Lactate (10 mM)

26 ± 8

Pyruvate (10 mM)

< 2

RelA -

 

Control

56 ± 12

D-glucose

< 2

SpoT -

 

Control

80 ± 6

D-Glucose

10 ± 3

CF5802

 

Control

62 ± 4

D-Glucose (10 mM)

< 2

CV2

 

Control

120 ± 11

D-glucose

< 2

L-lactate

< 2

  1. an. d., not determined
  2. The bacteria (A600 > 1) were incubated for 20 min at 37°C in minimal M9 medium containing substrates at the concentrations indicated. Mean ± SD for 3 - 9 experiments.