Figure 5
Dose-dependent effects of CCCP on AThTP content, respiration and growth of E. coli. (A) The bacteria (BL21 strain) were transferred to minimal M9 medium containing 10 mM D-glucose and the indicated CCCP concentrations. After 20 min (37°C, 250 rpm), the intracellular AThTP concentration was determined by HPLC. (B) Effect of CCCP on the respiratory ratio Γ (O2 consumption in the presence of CCCP over the O2 consumption in the absence of CCCP) measured in the presence of 10 mM glucose at 37°C by polarographic recording of O2 consumption. (C) Growth curves of the bacteria in the presence various concentrations of CCCP. (Means ± SD, n = 3)