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Table 3 Virulence (Mortality) to mouse of GT01Δnga with or without cloned nga gene

From: NADase as a target molecule of in vivo suppression of the toxicity in the invasive M-1 group A Streptococcal isolates

Strain

Mortalitya

NADaseb

GT01 (pLZ12-Km2, vector)

73% (8/11)

14.12 ± 1.30

GT01Δnga (pLZ12-Km2, vector)

0% (0/17)

0.04 ± 0.06

GT01Δnga (pLZN2, nga)

0% (0/11)

1.28 ± 0.12

GT01Δnga (pLZN-RBS, nga)

0% (0/10)

1.78 ± 0.03

GT01Δnga (pLZN-RBSII2, nga)

29% (4/14)

4.57 ± 0.17

  1. Bacteria were cultured in BHI-Y broth supplemented with kanamycin (100 μg/ml).
  2. a, Mice were observed for 8 days, because no mouse died after day 8 on previous study (see Figure 1).
  3. b, NADase activity was determined as described in Table 2.
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BMC Microbiology

ISSN: 1471-2180

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