Figure 2

Virulence (based on body weight change) to mouse of GT01 Δnga with or without cloned nga gene. (A) The change in body weight (% of the first weight) post-infection was shown in a week (* as a reference, the parental strain was shown in three days, because most mice died within this period). (B) Relationship of body weight and NADase activity was shown on days 2 and 3. NADase activities (0.04, 1.28, 1.78 and 4.57 U, respectively) of (a) GT01Δnga (pLZ12-km2), (b) GT01Δnga (pLZN2), (c) GT01Δnga (pLZN-RBS) and (d) GT01Δnga (pLZN-RBSII2) was plotted on the horizontal axis, respectively (see Table 3 or the text for NADase activity of each strain). The gradual increase in body weight (%) depended on higher NADase activity of the strains. The error bars indicate the standard error of the means.