Table 2 List of primers used in this study
From: Co-infection and localization of secondary symbionts in two whitefly species
Targeted gene | Primer name | Sequence (5'-> 3') | Anealing (°C)/Product Size | Reference |
|---|---|---|---|---|
Rickettsia 16S rDNA | Rb-F Rb-R | GCTCAGAACGAACGCTATC GAAGGAAAGCATCTCTGC | 59/~900 | [9] |
Hamiltonella 16S rDNA | 92F HbR | TGAGTAAAGTCTGGGAATCTGG AGTTCAAGACCGCAACCTC | 62/~700 | [10] |
Cardinium 16S rDNA | CFB-F CFB-R | GCGGTGTAAAATGAGCGTG ACCTMTTCTTAACTCAAGCCT | 59/~500 | [8] |
Arsenophonus 23S rDNA | Ars23S-1 Ars23S-2 | CGTTTGATGAATTCATAGTCAAA GGTCCTCCAGTTAGTGTTACCCAAC | 59/~600 | [5] |
Wolbachia 16S rDNA | Wol16S-f Wol16S-r | CGG GGGAAAAATTTATTGCT AGCTGTAATACAGAAAGTAAA | 55/~650 | [38] |
Fritschea 16S rDNA | U23F 23SIGR | GATGCCTTGGCATTGATAGGCGATGAAGGA TGGCTCATCATGCAAAAGGCA | 55/~600 | [7] |
B/Q biotypes mtCO1 | C1-J-2195 L2-N-3014 | TTGATTTTTTGGTCATCCAGAAGT TCCAATGCACTAATCTGCCATATTA | 50/~850 | [57] |
B/Q biotypes micro-sattelite | Bem23-F Bem23-R | CGGAGCTTGCGCCTTAGTC CGGCTTTATCATAGCTCTCGT | 55/Q = 400 B = 200 | [56] |