Portiera and Cardinium FISH of B. tabaci eggs, nymphs and adults. Portiera-specific probe (red) and Cardinium-specific probe (blue) were used. A, C and G: double FISH of Portiera and Cardinium in eggs (A), nymphs (D) and adults (G) under dark field. B, E and H: double FISH of Portiera and Cardinium in eggs (B), nymphs (E) and adults (H) under bright field. C, F and I are shown only with Cardinium probe to emphasize its location inside the bacteriosome.