Figure 5From: Photorhabdus adhesion modification protein (Pam) binds extracellular polysaccharide and alters bacterial attachmentComparison of bacterial attachment to surfaces in presence of insect hemolymph by fluorescence microscopy between TTO1rif and the pam mutant. Cells were grown on glass coverslips at 28°C for 8 hours. Planktonic bacteria were washed off and adherent bacteria were fixed and stained with DAPI. The adherence of TT01pam (B) is presented as a percentage of the data determined for the corresponding parental strain TT01rif (A). Bacterial counts were performed at 60× magnification and the data represent the mean values of 12 fields from triplicate experiments (± St.Dev) (C).Back to article page