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Table 3 Criteria of interpretation of biofilm production

From: N-acetylcysteine inhibit biofilms produced by Pseudomonas aeruginosa

Biofilm production average optical density (OD)
no biofilm producer ≤ ODc
weak biofilm producer ODc < ~ ≤ 2 × ODc
moderate biofilm producer 2 × ODc < ~ ≤ 4 × ODc
strong biofilm producer > 4 × ODc
  1. Note: optical density cut-off value (ODc) = average OD of negative control + 3 × SD of negative control.