Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis

Table 5 Primer sequences used for the relative quantification of glutamine synthetase and glutamate dehydrogenase genes.*

Gene	Sense Primer (5'-3')	Antisense Primer (5'-3')	Product size (bp)	Annealing Temperature (°C)
glnA1	ATGTGCTGCTGTTCAAGT	TGAAGGTGACGGTCTTGC	66	55
sigA	GACTCGGTTCGCGCCTA	CCTCTTCTTCGGCGTTG	64	55
msmeg_6272	TGATCCGCCACATCCTG	GATGTAGGTGCCGATGC	65	56.5
msmeg_5442	AGATCATGCGGTTCTGTC	GTGTATTCACCGATGTGCC	61	55
msmeg_4699	GTGAGGACTTCCGCACC	CCGCTTGACGACGAATC	104	55

*The product size and annealing temperatures are also given. sigA was used as an internal control or housekeeping gene. Reverse transcriptase PCR reactions were carried out in the GeneAmp PCR System 9700

ISSN: 1471-2180