Figure 3

Reverse transcriptase PCR of msmeg_6272 cultured under conditions of nitrogen starvation (3 mM (NH ₄ ) ₂ SO ₄ ) for four hours. Lane (1) 0 hr at which point M. smegmatis was exposed to nitrogen excess (60 mM (NH₄)₂SO₄) for 1 hr (2) 0.5 hr nitrogen starvation; (3) 1 hr nitrogen starvation (4) 2 hrs nitrogen starvation and (5) 4 hrs nitrogen starvation. SigA was amplified as an unregulated internal control.