Figure 7
LaTRF interacts with telomeric DNA in vivo. Chromatin immunoprecipitation (CHIP) of mid-log phase promastigotes cells using anti-LaTRF. Control experiments were done with chromatin immunoprecipitated in the presence of pre-immune serum and without serum (mock). Total DNA (input) corresponds to 10% and 1% of the amount of DNA in 108 cells cross-linked with the chromatin. Slot-blots were hybridized with 5' end-labeled Tel1 probe (left) and re-hybridized with the kDNA probe (right).