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Figure 5 | BMC Microbiology

Figure 5

From: The Leishmania amazonensis TRF (TTAGGG repeat-binding factor) homologue binds and co-localizes with telomeres

Figure 5

Supershift and competition assays confirm that recombinant full length LaTRF bind in vitro double-stranded telomeric DNA. Electrophoretic mobility shift assays (EMSA) were done using radiolabeled double-stranded telomeric DNA (LaTEL) as probe. Protein:DNA complexes were separated in a 4% PAGE in 1X TBE. EMSA was done with recombinant full length LaTRF and anti-LaTRF serum in the absence (lane 2) and in the presence of 20 fold excess of non-labeled LaTEL as specific competitor (lane 3) or 100 fold excess of double-stranded non-specific DNA (poly [dI-dC] [dI-dC]) as non specific competitor (lane 4). In lane 1 reaction was done in the presence of LaTEL only.

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