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Table 3 Growth rate and final cell density of different Z. mobilis strains in the absence or presence of different pretreatment inhibitors.

From: The Zymomonas mobilis regulator hfq contributes to tolerance against multiple lignocellulosic pretreatment inhibitors

  

ZM4

AcR

AcRIM0347

AcRIM0347(p42-0347)

Growth rate (hour -1 )

RM

0.48 ± 0.03

0.46 ± 0.003

0.35 ± 0.004

0.32 ± 0.003

 

HMF

0.36 ± 0.02

0.35 ± 0.01

0.19 ± 0.02

0.22 ± 0.001

 

Furfural

0.31 ± 0.01

0.30 ± 0.005

0.19 ± 0.03

0.20 ± 0.01

 

Vanillin

0.26 ± 0.001

0.26 ± 0.01

0.20 ± 0.006

0.20 ± 0.003

Final Cell Density (OD 600 nm )

RM

0.91 ± 0.01

0.98 ± 0.006

0.95 ± 0.003

0.92 ± 0.006

 

HMF

0.93 ± 0.003

0.96 ± 0.006

0.67 ± 0.03

0.78 ± 0.02

 

Furfural

0.88 ± 0.006

0.89 ± 0.009

0.67 ± 0.001

0.80 ± 0.02

 

Vanillin

0.69 ± 0.006

0.71 ± 0.01

0.66 ± 0.01

0.70 ± 0.01

  1. The concentration for the inhibitor supplemented into the RM is: HMF: 0.75 g/L, furfural, or vanillin: 1 g/L. Strains included in this study are: ZM4: Zymomonas mobilis ZM4 wild-type; AcR: previously described ZM4 acetate tolerant mutant; AcRIM0347: AcR insertional mutant of ZMO0347; AcRIM0347 (p42-0347): AcRIM0347 containing gateway plasmid p42-0347. This experiment has been repeated at least three times with similar result. Duplicate biological replicates were used for each condition.