Characterization of growth of the sur7 Δ null mutant strain. The growth of strains DAY185 (black), sur7 Δ (red), and sur7 Δ+SUR7 (blue) were compared under different conditions. Standard growth conditions at 30°C in complete synthetic medium (CSM) supplemented with uridine is shown in (A). Growth at extreme temperature (42°C) was also tested in CSM supplemented with uridine (B) and CSM with 1 M NaCl supplemented with uridine (C). At high temperatures (42°C), the impaired growth of the sur7 Δ null mutant strain was statistically significant compared to the control strains. All growth curves were performed in triplicate, with Log10 of OD600 plotted against Time (hours). Corresponding error bars are indicated. Table 2 lists the calculated doubling time of each strain under each condition presented here.