Figure 1

Characterization of growth of the sur7 Δ null mutant strain. The growth of strains DAY185 (black), sur7 Δ (red), and sur7 Δ+SUR7 (blue) were compared under different conditions. Standard growth conditions at 30°C in complete synthetic medium (CSM) supplemented with uridine is shown in (A). Growth at extreme temperature (42°C) was also tested in CSM supplemented with uridine (B) and CSM with 1 M NaCl supplemented with uridine (C). At high temperatures (42°C), the impaired growth of the sur7 Δ null mutant strain was statistically significant compared to the control strains. All growth curves were performed in triplicate, with Log₁₀ of OD₆₀₀ plotted against Time (hours). Corresponding error bars are indicated. Table 2 lists the calculated doubling time of each strain under each condition presented here.