Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv

Table 2 List of the 14 most frequently observed membrane proteins.

Sanger ID	Gene name	Protein identity	No. of TMH ^a	No. of observed peptides ^b	emPAI (Mol %) ^c	References
Rv1078	pra	Possible proline rich antigen	2	5	5.66	[14, 24]
Rv1489	-	Conserved hypothetical protein	2	5	1.30	[26]
Rv1306	atpF	Possible ATP synthase b chain	1	7	0.36	[14, 24–26]
Rv2563	-	Possible glutamine-transport transmembrane protein	4	13	0.35	[14, 25, 26, 32]
Rv1234	-	Possible transmembrane protein	2	7	0.26	[25, 26]
Rv0072	-	Possible glutamine-transport transmembrane protein	4	11	0.23	[25, 26]
Rv0479c	-	Possible conserved membrane protein	1	11	0.23	[24–26]
Rv2969c	-	Possible conserved membrane or secreted protein	1	11	0.19	[14, 24–26, 40]
Rv2200c	ctaC	Possible transmembrane cytochrome C oxidase	3	13	0.17	[14, 24–26, 32]
Rv2195	qcrA	Possible rieske iron-sulfur protein	3	15	0.16	[14, 24–26, 40, 54]
Rv1223	htrA	Possible serine protease	1	19	0.15	[24, 26, 54]
Rv1822	-	Phosphatidylglycerophosphate synthase	4	5	0.14	[14]
Rv2721c	-	Possible conserved transmembrane protein	2	12	0.13	[14, 24–26, 32]
Rv3273	-	Possible transmembrane carbonic anhydrase	10	11	0.11	[24–26, 54]

^a Number of TMH regions predicted by TMHMM version 2.0 publically available at http://www.cbs.dtu.dk/services/TMHMM/.
^b Number of observed unique peptides from each protein.
^c Relative protein abundance provided in mol % concentration.

ISSN: 1471-2180