Effects of apples and specific apple components on the cecal environment of conventional rats: role of apple pectin

Table 6 Primers used for Real-Time PCR

Target gene	Forward primer (5'-3')	Reverse primer (5'-3')	Product size (bp)	PCR Efficiency (%)	Correlation coefficient (R²)	Reference
Clostridium coccoides 16S	aaa tga cgg tac ctg act aa	ctt tga gtt tca ttc ttg cga a	440	97,8	0,998	[43]
Bifidobacterium 16S	cgc gtc ygg tgt gaa ag	ccc cac atc cag cat cca	244	93,0	0,995	[44]
Lactobacillus 16S	agcagtagggaatcttcca	caccgctacacatggag	341	98,6	0,998	[45, 46]
Bacteroides spp.16S^a	cgg cga aag tcg gac taa ta	acg gag tta gcc gat gct ta	360	100,1	0,997	This study
Butyryl-Coenzyme A	gcn gan cat ttc acn tgg aay wsn tgg cay atg	cct gcc ttt gca atr tcn acr aan gc	530	97,5	0,965	[21]
V2-V3 16S region (HDA)^b	act cct acg gga ggc agc agt	gta tta ccg cgg ctg ctg gca c	200	113,7	0,991	[40]

^aThe bacteroides primer set was designed to amplify a segment of the DNA sequence represented by the highly homologous bands 4-7 in Table 5. ^bPCR for the HDA primer set was run in parallel for each set of primers for all samples.