Skip to main content

Table 2 Effects of bacterial cell-derived immune elicitors on susceptibility of third-instar gypsy moth larvae reared without enteric bacteria (antibiotics) or with enteric bacteria (no antibiotics) to B. thuringiensis (Bt).

From: Chemical modulators of the innate immune response alter gypsy moth larval susceptibility to Bacillus thuringiensis

a) Bt cell preparation (DiPel, 50 IU)
   Reared without antibiotics Reared with antibiotics
Rearing treatment Elicitor added to B. thuringiensis Bt alone Bt alone
No Antibiotics Bt alone -- < 0.0001
No Antibiotics Enterobacter sp. NAB3 0.6882 < 0.0001
Antibiotics Enterobacter sp. NAB3 0.0956 < 0.0001
No Antibiotics Crude lipopolysaccharide 0.8231 < 0.0001
Antibiotics Crude lipopolysaccharide 0.0001 0.4942
No Antibiotics Purified lipopolysaccharide 0.7268 < 0.0001
Antibiotics Purified lipopolysaccharide < 0.0001 0.5731
No Antibiotics Bacillus cereus peptidoglycan 0.0582 0.0100
Antibiotics Bacillus cereus peptidoglycan 0.0065 0.7331
No Antibiotics Vibrio fisheri peptidoglycan 0.1092 < 0.0001
Antibiotics Vibrio fisheri peptidoglycan 0.0010 0.1276
No Antibiotics Tracheal cytotoxin 0.0539 < 0.0001
Antibiotics Tracheal cytotoxin 0.4070 < 0.0001
No Antibiotics Lysozyme-digested V. fisheri peptidoglycan 0.2622 < 0.0001
Antibiotics Lysozyme-digested V. fisheri peptidoglycan 0.2356 < 0.0001
No Antibiotics Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide 0.1120 < 0.0001
Antibiotics Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide 0.2328 0.0002
b) Bt Cry1Ac toxin (MVPII, 20 ug)
   Reared without antibiotics Reared with antibiotics
Rearing treatment Elicitor added to B. thuringiensis Bt alone Bt alone
No Antibiotics Bt alone -- 0.0202
No Antibiotics Enterobacter sp. NAB3 < 0.0001 < 0.0001
Antibiotics Enterobacter sp. NAB3 0.7182 0.0002
No Antibiotics Crude lipopolysaccharide 0.6689 0.0919
Antibiotics Crude lipopolysaccharide 0.0440 0.8517
No Antibiotics Purified lipopolysaccharide 0.8138 0.0038
Antibiotics Purified lipopolysaccharide 0.0456 0.5915
No Antibiotics Bacillus cereus peptidoglycan 0.0651 < 0.0001
Antibiotics Bacillus cereus peptidoglycan 0.0264 0.1951
No Antibiotics Vibrio fisheri peptidoglycan 0.5111 0.0056
Antibiotics Vibrio fisheri peptidoglycan 0.0196 0.8623
No Antibiotics Tracheal cytotoxin 0.9977 0.0116
Antibiotics Tracheal cytotoxin 0.0188 0.8914
No Antibiotics Lysozyme-digested V. fisheri peptidoglycan < 0.0001 < 0.0001
Antibiotics Lysozyme-digested V. fisheri peptidoglycan 0.7613 0.0001
No Antibiotics Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide 0.0005 < 0.0001
Antibiotics Lysozyme-digested V. fisheri peptidoglycan + purified lipopolysaccharide 0.5645 < 0.0001
  1. Two formulations of B. thuringiensis, DiPel 50 IU (a) and MVPII 20 μg (b), were assayed. The significance (p-values) of the log-rank test comparing larval mortality of each experimental treatment group to Bt alone or Bt alone when reared with antibiotics is shown.