Delineation of regulatory region. Deletion constructs were generated to segregate promoter and the regulatory regions of IGPr. The column labeled as construct shows configuration of the inserts in different clones used in transformation of M.smegmatis mc2 155. The numbering is with reference to the translational initiation signal for Rv0167 as +1. The mutation in VPCI591 is shown as a filled triangle, the regions deleted in each clone is indicated by delta symbol. IGPr: 200 bp intergenic region between Rv0166 and Rv0167.