Figure 2

Replication of DENV in Drosophila melanogaster S2 cells. A: Titer of 12 strains of DENV 5 days post infection following passage 1 (S2 p1 MOI 10, solid bars) and passage 2 (S2 p2 MOI 10, open bars) in Drosophila melanogaster S2 cells. In passage 1, cells were infected with each virus strain at MOI 10. In passage 2, cells were infected with 500 μl of cell supernatant from passage 1; B: Titer of 12 strains of DENV 5 days pi following infection of S2 cells at MOI 0.1 (S2 p1 MOI 0.1); C: Replication kinetics of DENV-4 Taiwan at MOI 0.1 in Drosophila melanogaster S2 cells.