Propionibacterium acnes infection induces upregulation of inflammatory genes and cytokine secretion in prostate epithelial cells

Table 1 List of genes that are upregulated upon P. acnes infection.

Gene name	Description	Fold upregulation
CCL2	Chemokine (C-C motif) ligand 2	41
CSF2	Colony stimulating factor 2 (granulocyte-macrophage)	139
CSF3	Colony stimulating factor 3 (granulocyte)	39
CXCL10	Chemokine (C-X-C motif) ligand 10	107
IFNB1	Interferon, beta 1, fibroblast	12
IL1A	Interleukin 1, alpha	12
IL6	Interleukin 6 (interferon, beta 2)	34
IL8	Interleukin 8	336
IRAK2	Interleukin-1 receptor-associated kinase 2	11
IRF1	Interferon regulatory factor 1	12
JUN	Jun oncogene	10
LTA	Lymphotoxin alpha (TNF superfamily, member 1)	5
NFKB2	Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)	8
NFKBIA	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha	6
REL	V-rel reticuloendotheliosis viral oncogene homolog	4
RELA	V-rel reticuloendotheliosis viral oncogene homolog A,	2
RIPK2	Receptor-interacting serine-threonine kinase 2	4
TLR2	Toll-like receptor 2	3
TNF	Tumor necrosis factor (TNF superfamily, member 2)	53
TICAM1	Toll-like receptor adaptor molecule 1	3

Semiconfluent RWPE-1 monocell-layers were infected with P. acnes at a MOI of 16:1. After 24 h infection, the cells were harvested, mRNA was collected and cDNA was prepared. The cDNA corresponding to 84 inflammation-associated genes was quantified with qRT-PCR and compared with cDNA prepared from non-infected cells. Inclusion criteria: > 2-fold up-regulation, (p = 0.05).

ISSN: 1471-2180