CLSM images of monospecies ( Candida spp . or P. aeruginosa ) and dual species ( Candida spp . and P. aeruginosa ) biofilms. (A). Adhesion of C. albicans for 90 min, (B). Adhesion of C. albicans and P. aeruginosa for 90 min, (C). Adhesion of P. aeruginosa for 90 min. Note the mutual inhibition of adhesion of both pathogens in dual species environment. (D) Initial colonization of C. dubliniensis for 24 h (E). Initial colonization of C. dubliniensis and P. aeruginosa for 24 h, (F). Initial colonization of P. aeruginosa for 24 h. Note the impaired biofilm formation after 24 h in the dual species biofilm due to mutual inhibition of these organisms. (G) Maturation of C. tropicalis for 48 h, (H). Maturation of C. tropicalis and P. aeruginosa for 48 h, (I). maturation of P. aeruginosa for 48 h. Note the altered and scant biofilm maturation in dual species biofilm as a result of mutual inhibition of C. tropicalis and P. aeruginosa.