Figure 1
From: Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
Comparison of the hemolytic activity of MFN1032 and CHA and characterization of MFN1032 cell-associated hemolysis. Hemolysis of RBCs (% HA) incubated with MFN1032 and CHA, at 37°C and with a multiplicity of infection (MOI) of 1. Cells were subjected or not to centrifugation at 1500 g or 400 g for 10 min to enhance cell-cell contact. cHA indicates cell-associated hemolytic activity and sHA indicates secreted hemolytic activity. MFN1032 sup indicates MFN1032 cell-free supernatant. MFN1032 stat indicates MFN1032 cells in stationary growth phase. MFN1032 sup lysis indicates supernatants obtained after RBC lysis by MFN1032. Hemolytic activity was measured as described in the materials and methods. Results are means of at least three independent experiments. Standard deviation is shown.