Figure 3

Az inhibition of intracellular Francisella strains. After 22 hours, recovered bacterial counts were measured for F. philomiragia, F. novicida, and F. tularensis LVS infected cells (MOI 500). A) J774A.1 cells infected with F. philomiragia, F. novicida, or F. tularensis LVS had more than 10⁵ CFU/ml. Bacterial counts decreased for all strains as the Az concentrations increased and were near 0 CFU/ml at 5 μg/ml Az. B) A549 cells infected with F. philomiragia, F. novicida, or F. tularensis LVS had more than 10⁵ CFU/ml at 0 μg/ml Az. Bacterial counts decreased at 0.1 and 5 μg/ml Az and were near 0 CFU/ml at 25 μg/ml Az. CFU counts from no Az treatment compared 0.1, 5, and 25 μg/ml Az treatment for all Francisella strains were significantly different (p-value < 0.005).