Table 2 Forward (FW) and reverse (RV) primers used in real-time PCR for the PLB and LIP genes.
Gene | Orientation | Primer sequence (5' to 3') |
|---|---|---|
PLB1 | FW | GGTGGAGAAGATGGCCAAAA |
RV | AGCACTTACGTTACGATGCAACA | |
PLB2 | FW | TGAACCTTTGGGCGACAACT |
RV | GCCGCGCTCGTTGTTAA | |
LIP1 | FW | AGCCCAACCAGAAGCTAATGAA |
RV | TGATGCAAAAGTCGCCATGT | |
LIP2 | FW | GGCCTGGATTGATGCAAGAT |
RV | TTGTGTGCAGACATCCTTGGA | |
LIP3 | FW | TCTCACCGAGATTGTTGTTGGA |
RV | GTTGGCCATCAAATCTTGCA | |
LIP4 | FW | GCGCTCCTGTTGCTTTGACT |
RV | ACACGGTTTGTTTTCCATTGAA | |
LIP5 | FW | TGGTTCCAAAAATACCCGTGTT |
RV | CGACAATAGGGACGATTTGATCA | |
LIP6 | FW | AAGAATCTTCCGACCTGACCAA |
RV | ATATGCACCTGTTGACGTTCAAA | |
LIP7 | FW | AACTGATATTTGCCATGCATTAGAAA |
RV | CCATTCCCGGTAACTAGCATGT | |
LIP8 | FW | CAACAATTGCTAAAATCGTTGAAGA |
RV | AGGGATTTTTGGCACTAATTGTTT | |
LIP9 | FW | CGCAAGTTTGAAGTCAGGAAAA |
RV | CCCACATTACAACTTTGGCATCT | |
LIP10 | FW | CACCTGGCTTAGCAGTTGCA |
RV | CCCAGCAAAGACTCATTTTATTCA |