Figure 2

Growth of H. influenzae strains R2846, HI1380 and HI1390 and their corresponding isogenic fhuD insertion mutant derivatives with ferric ferrichrome as the sole iron source. Growth of all strains is in either hdBHI supplemented with heme as the sole heme and iron source or in hdBHI supplemented with protoporphyrin IX as a porphyrin source, EDDA to chelate free iron and ferric ferrichrome as the sole iron source. (A) Wildtype strain R2846 with heme at 10 μg ml^-1 (solid circles) and with ferric ferrichrome at 200 μM (solid triangles). The fhuD insertion mutant strain HI2128 with heme at 10 μg ml^-1 (open circles) and with ferric ferrichrome at 200 μM (open triangles). (B) Wildtype strain HI1380 with heme at 10 μg ml^-1 (solid circles) and with ferric ferrichrome at 200 μM (solid triangles). The fhuD insertion mutant strain HI2131 with heme at 10 μg ml^-1 (open circles) and with ferric ferrichrome at 200 μM (open triangles). (C) Wildtype strain HI1390 with heme at 10 μg ml^-1 (solid circles) and with ferric ferrichrome at 200 μM (solid triangles). The fhuD insertion mutant strain HI2132 with heme at 10 μg ml^-1 (open circles) and with ferric ferrichrome at 200 μM (open triangles). Results are mean ± SD for quintuplicate results from representative experiments.