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Table 1 Effect of a tenfold dilution series of prothioconazole, prothioconazole + fluoxastrobin and azoxystrobin on fungal biomass measured by Q-PCR analysis.

From: Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum

 

prothio

prothio+catalase*

prothio+fluoxa

prothio+fluoxa+catalase*

azoxy

azoxy+catalase*

control

235.68a

194.60a

255.68a

245.89a

251.57a

232.45a

1/1000

9.42b

63.03b

76.23b

48.17b

267.16a

230.12a

1/100

2.35c

31.13c

16.58c

44.90b

250.01a

234.93a

1/10

2.51c

50.02bc

LD

LD

254.22a

216.00a

field

LD

33.47c

LD

LD

236.54a

170.72a

  1. F. graminearum biomass expressed as ng/ÎĽl. In each run, a no-template control was included. The amount of fungal material was measured based on a standard series of F. graminearum DNA ranging from 100 ng/ÎĽl down to 3.125 ng/ÎĽl which was carried out in triplicate.
  2. Different letters indicate significant differences after analysis with a Kruskall-Wallis Mann-Whitney analysis with P = 0.05
  3. Prothio: prothioconazole; azoxy: azoxystrobin; fluoxa:fluoxastrobin
  4. *: Effect of catalase (1000 U/ml) added at the start of the experiment on the F. graminearum biomass.
  5. LD: Lower than detection limit.