Figure 4

Effect of prothioconazole + fluoxastrobin (a, c) and prothioconazole (b, d) in absence (dashed line) or presence (solid line) of exogenous catalase on the germination of F. graminearum conidia after 4 h (a, b) and 48 h (c,d). Conidia at a concentration of 10e⁶ were challenged with a tenfold dilution series of fluoxastrobin + prothioconazole, azoxystrobin and prothioconazole starting from 0.5 g/l + 0.5 g/l, 0.83 g and 0.67 g/l. At the beginning of the experiment, catalase (1000 U/ml) was added to the germinating conidia. For each treatment and repetition 50 conidia were scored for their germination after staining with 0.02% of cotton blue in lactic acid and percentage of conidial germination was calculated. This experiment was repeated twice in time. Different letters at each data point indicate differences from the control treatment after analysis with a Kruskall-Wallis and Mann-Whitney test with a sequential Bonferroni correction for multiple comparisons.