Table 1 Primers used for RealTime-PCR
Primer | DNA sequence (5' → 3') | Application | Amplicon |
|---|---|---|---|
spaP-Fw | TCCGCTTATACAGGTCAAGTTG | spaP fragment | 121 bp |
spaP-Rv | GAGAAGCTACTGATAGAAGGGC | Â | Â |
gtfB-Fw | AGCAATGCAGCCATCTACAAAT | gtfB fragment | 98 bp |
gtfB-Rv | ACGAACTTTGCCGTTATTGTCA | Â | Â |
gbpB-Fw | CGTGTTTCGGCTATTCGTGAAG | gbpB fragment | 108 bp |
gbpB-Rv | TGCTGCTTGATTTTCTTGTTGC | Â | Â |
luxS-Fw | ACTGTTCCCCTTTTGGCTGTC | luxS fragment | 93 bp |
luxS-Rv | AACTTGCTTTGATGACTGTGGC | Â | Â |
brpA-Fw | CGTGAGGTCATCAGCAAGGTC | brpA fragment | 148 bp |
brpA-Rv | CGCTGTACCCCAAAAGTTTAGG | Â | Â |
ldh-Fw | TTGGCGACGCTCTTGATCTTAG | ldh fragment | 92 bp |
ldh-Rv | GTCAGCATCCGCACAGTCTTC | Â | Â |